Though we haven't tested PneumaCult™ media for use with murine epithelial cells, there are publications that report this application.
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Evaluate the probable poisonous results of candidate therapeutics, such as little molecule compounds and biologics.
Operate swiftly at this step to pool wells right into a fifteen mL tube. Notice: For a big-scale dissociation, use a multichannel pipette to pool cells right into a sterile reagent reservoir. Incorporate the pooled wells into a fifteen mL tube.
See information showing remarkable ALI tradition morphology and higher epithelial cell marker expression utilizing these advisable Transwell® inserts >
Whilst we have not directly analyzed nasal epithelial cells in-house, there are publications that report PneumaCult™ supporting this cell type.
Evaluate the status by checking a droplet and stopping the dissociation when all over eighty% of your cell suspension is single-celled. Steer clear of above-digestion.
Here's a list of revealed literature that cites culturing of nasal epithelial cells working with PneumaCult™ culture media:
Just in case cell clumping is observed soon after thawing cryopreserved cells, it is suggested to filter aggregated suspensions via a 37 µm cell strainer (e.g. Catalog #27250) for ideal final results. In this instance, the operate time over the CellPore™ Transfection System could have to be greater to ten seconds.
To assess the regional specificity from the little vs large airway, you could conduct the following assays:
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A minimum response quantity of fifty µL is necessary for dependable overall performance With all the CellPore™ Transfection System.
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